Currently the Bulgarian center for advanced microscopy includes the following equipment:

  1. Andor Revolution XD spinning disc confocal microscope system for high-resolution live cell imaging, equipped with FRAP and laser micro-irradiation, that includes:
    1. Nikon TiE Inverted Microscope with Autofocus System
    2. Yokogawa CSU-X1 spinning disk
    3. Microscope stage automated in X, Y and Z directions. Movement in the Z direction is accomplished by a piezo element
    4. Three solid state lasers with wavelengths of 405 nm, 488 nm and 561 nm
    5. FRAPPA unit for Fluorescence recovery after photobleaching (FRAP) and photoactivation (PA)
    6. TuCam module that allows simultaneous imaging of two different wavelengths
    7. Two iXon3 897 EMCCD Cameras
    8. Borealis
    9. Optical anti-vibration table
    10. Computer configuration for controlling the microscope system
    11. Cell incubator for the microscope
    12. CO2 controller that maintains proper CO2 concentration in the cell incubator
    13. Temperature controller that maintains proper temperature in the cell incubator
    14. Micropoint laser micro-irradiation module
    15. IQ3 software for controlling the components of the microscope system, spinning disk, micro-irradiation module and FRAP module
    16. MetaMorph software for controlling the components of the microscope system and for image analysis
  2. Zeiss Axiovert 200M inverted motorized epifluorescence microscope that includes:
    1. Axiovert 200M inverted motorized epifluorescence microscope
    2. Apotom
    3. Axiocam camera
    4. Anti-vibration table
    5. Axiovision, software for controlling the components of the microscope system and for image analysis
  3. Andor Dragonfly 505 microscopy system for:
    1. Multi-Point Confocal, Widefield and TIRF microscopy
    2. ClearView-GPU Deconvolution, SRRF-Stream super-resolution, dSTORM super-resolution
    3. Andor Mosaic 3 Duet for FRAP и photomanipulation
    4. Andor MicroPoint Galvo – laser micro-irradiation
    5. Andor ILE-400 laser combiner with Borealis
    6. 405 nm, 488 nm, 561 nm, 637 nm solid-stаte lasers
    7. Okolab Bold Line Cage CO2 incubator
    8. Inverted microscope ECLIPSE Ti2-E
    9. Perfect Focus 4 autofocus system
    10. Andor iXon Ultra 888 EMCCD camera
    11. Andor Zyla 4.2 Plus CMOS camera
    12. CoolLED pE300 epi-fluorescents for DAPI, GFP, RFP
    13. CoolLED pE100
    14. Fusion software package
    15. IQ3 software package
  4. STELLARIS 8 STED Microscopes
    1. Laser confocal scanning microscopy
    2. White laser (excitation from 440 nm up to 790 nm)
    3. Power HyD S, HyD X, and HyD R detectors
    4. Stimulated emission depletion (STED) super-resolution microscopy
    5. FALCON: Fluorescence lifetime imaging microscopy (FLIM)
    6. TauSTED Stimulated emission depletion microscopy with FLIM
    7. Fluorescence Correlation Spectroscopy (FCS)
    8. Fluorescence Resonance Energy Transfer (FRET) Microscopy
    9. Lightning (adaptive deconvolution)
    10. FRAP
    11. Optical anti-vibration table
    12. Computer configuration for controlling the microscope system
    13. Cell incubator for the microscope
    14. CO2 and O2 controller that maintains proper CO2 and O2 concentration in the cell incubator
    15. Temperature controller that maintains proper temperature in the cell incubator
    16. Leica Application Suite X (LAS X) software
  5. ZEISS LSM 980 with Airyscan 2
    1. laser confocal scanning microscopy
    2. 36 simultaneous channels (GASP) detector
    3. 405, 488, 561 and 594 laser lines.
    4. airyscan super-resolution
    5. FRAP
    6. UV micro-irradiation
    7. Optical anti-vibration table
    8. Computer configuration for controlling the microscope system
    9. Cell incubator for the microscope
    10. CO2 and O2 controller that maintains proper CO2 and O2 concentration in the cell incubator
    11. Temperature controller that maintains proper temperature in the cell incubator
    12. Zen blue software
  6. PicoQuant FLIM for ZEISS LSM 980
    1. Rapid FLIM HiRes
    2. 488 and 561 pulse lasers
    3. PMA Hybrid detector and SPAD (single photon avalanche diode) detector.
    4. TCSPC Electronics (MultiHarp 150)
    5. Pulsed Interleaved Excitation (PIE)
    6. Fluorescence Anisotropy (Polarization)
    7. Fluorescence Correlation Spectroscopy (FCS)
    8. Fluorescence Resonance Energy Transfer (FRET) Microscopy
    9. SymPhoTime 64 Analysis software
  7. ZEISS SteREO Discovery.V20
  8. Image analysis software:
    1. Imaris
    2. CellTool
    3. ImageJ
    4. Fiji