Currently the Bulgarian center for advanced microscopy includes the following equipment:
- Andor Revolution XD spinning disc confocal microscope system for high-resolution live cell imaging, equipped with FRAP and laser micro-irradiation, that includes:
- Nikon TiE Inverted Microscope with Autofocus System
- Yokogawa CSU-X1 spinning disk
- Microscope stage automated in X, Y and Z directions. Movement in the Z direction is accomplished by a piezo element
- Three solid state lasers with wavelengths of 405 nm, 488 nm and 561 nm
- FRAPPA unit for Fluorescence recovery after photobleaching (FRAP) and photoactivation (PA)
- TuCam module that allows simultaneous imaging of two different wavelengths
- Two iXon3 897 EMCCD Cameras
- Borealis
- Optical anti-vibration table
- Computer configuration for controlling the microscope system
- Cell incubator for the microscope
- CO2 controller that maintains proper CO2 concentration in the cell incubator
- Temperature controller that maintains proper temperature in the cell incubator
- Micropoint laser micro-irradiation module
- IQ3 software for controlling the components of the microscope system, spinning disk, micro-irradiation module and FRAP module
- MetaMorph software for controlling the components of the microscope system and for image analysis
- Zeiss Axiovert 200M inverted motorized epifluorescence microscope that includes:
- Axiovert 200M inverted motorized epifluorescence microscope
- Apotom
- Axiocam camera
- Anti-vibration table
- Axiovision, software for controlling the components of the microscope system and for image analysis
- Andor Dragonfly 505 microscopy system for:
- Multi-Point Confocal, Widefield and TIRF microscopy
- ClearView-GPU Deconvolution, SRRF-Stream super-resolution, dSTORM super-resolution
- Andor Mosaic 3 Duet for FRAP и photomanipulation
- Andor MicroPoint Galvo – laser micro-irradiation
- Andor ILE-400 laser combiner with Borealis
- 405 nm, 488 nm, 561 nm, 637 nm solid-stаte lasers
- Okolab Bold Line Cage CO2 incubator
- Inverted microscope ECLIPSE Ti2-E
- Perfect Focus 4 autofocus system
- Andor iXon Ultra 888 EMCCD camera
- Andor Zyla 4.2 Plus CMOS camera
- CoolLED pE300 epi-fluorescents for DAPI, GFP, RFP
- CoolLED pE100
- Fusion software package
- IQ3 software package
- STELLARIS 8 STED Microscopes
- Laser confocal scanning microscopy
- White laser (excitation from 440 nm up to 790 nm)
- Power HyD S, HyD X, and HyD R detectors
- Stimulated emission depletion (STED) super-resolution microscopy
- FALCON: Fluorescence lifetime imaging microscopy (FLIM)
- TauSTED Stimulated emission depletion microscopy with FLIM
- Fluorescence Correlation Spectroscopy (FCS)
- Fluorescence Resonance Energy Transfer (FRET) Microscopy
- Lightning (adaptive deconvolution)
- FRAP
- Optical anti-vibration table
- Computer configuration for controlling the microscope system
- Cell incubator for the microscope
- CO2 and O2 controller that maintains proper CO2 and O2 concentration in the cell incubator
- Temperature controller that maintains proper temperature in the cell incubator
- Leica Application Suite X (LAS X) software
- ZEISS LSM 980 with Airyscan 2
- laser confocal scanning microscopy
- 36 simultaneous channels (GASP) detector
- 405, 488, 561 and 594 laser lines.
- airyscan super-resolution
- FRAP
- UV micro-irradiation
- Optical anti-vibration table
- Computer configuration for controlling the microscope system
- Cell incubator for the microscope
- CO2 and O2 controller that maintains proper CO2 and O2 concentration in the cell incubator
- Temperature controller that maintains proper temperature in the cell incubator
- Zen blue software
- PicoQuant FLIM for ZEISS LSM 980
- Rapid FLIM HiRes
- 488 and 561 pulse lasers
- PMA Hybrid detector and SPAD (single photon avalanche diode) detector.
- TCSPC Electronics (MultiHarp 150)
- Pulsed Interleaved Excitation (PIE)
- Fluorescence Anisotropy (Polarization)
- Fluorescence Correlation Spectroscopy (FCS)
- Fluorescence Resonance Energy Transfer (FRET) Microscopy
- SymPhoTime 64 Analysis software
- ZEISS SteREO Discovery.V20
- Image analysis software:
- Imaris
- CellTool
- ImageJ
- Fiji